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. Author manuscript; available in PMC: 2017 Dec 15.
Published in final edited form as: Cell. 2016 Dec 15;167(7):1719–1733.e12. doi: 10.1016/j.cell.2016.11.052

Figure 6. Amelioration of Aging Hallmarks in Wild-Type Mice and Human Cells by Short-Term In Vitro Induction of Oct4, Sox2, Klf4, and c-Myc.

Figure 6

(A) Immunofluorescence of Oct4 and Sox2 in WT 4F TTFs. Scale bar, 5 μm.

(B) Immunofluorescence and quantification of γH2AX foci in late-passage cells from WT 4F mice. Scale bar, 10 μm. ****p < 0.0001 according to one-way ANOVA with Bonferroni correction.

(C) qPCR analysis of stress response genes in the p53 pathway, senescence-associated metalloprotease MMP13, and interleukin-6 in late-passage cells from WT 4F mice. *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001 according to one-way ANOVA with Bonferroni correction.

(D) Immunofluorescence and quantification of H3K9me3 in late-passage WT 4F cells. Scale bar, 10 μm. *p < 0.05, and ****p < 0.0001 according to one-way ANOVA with Bonferroni correction compared to control.

(E) Immunofluorescence and quantification of γH2AX foci in late-passage human 4F cells. Scale bar, 10 μm. ***p < 0.0005 and ****p < 0.0001 according to one-way ANOVA with Bonferroni correction compared to control.

(F) Immunofluorescence and quantification of H3K9me3 in late-passage human 4F cells. Scale bar, 10 μm. *p < 0.05, **p < 0.005, and ****p < 0.0001 according to one-way ANOVA with Bonferroni correction compared to control.

Data are presented as mean ± SEM. See also Figure S6 and Table S1.