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. Author manuscript; available in PMC: 2018 Nov 1.
Published in final edited form as: J Immunol. 2017 Oct 2;199(9):3187–3201. doi: 10.4049/jimmunol.1700851

FIGURE 1.

FIGURE 1

HLA-DR7-restricted HCMV glycoprotein B DYS-epitope specific CD4+ T cells undergo memory inflation in HIV+ HLA-DR7+ subjects. A–B: CD4-enriched or untouched peripheral blood mononuclear cells (PBMCs) from HIV+ DR7+ subjects were stained with DR7:CLIP or DR7:DYS tetramer for their (A) tp1 (no ART) (n=8), and (B) tp2 (on ART) samples (n=4). (C) DR7:DYS tetramer staining of HIV− DR7+ subjects’ PBMCs (n=10). (D) Response magnitude comparisons of DYS+ and EPD+ CD4+ T cells from HIV+ HCMV+ DR7+ (n=8; tp1 of Subject 10013, and tp2 of Subjects 10004, 10027 and 10032), HIV− HCMV+ DR7+ (n=10) and HIV+ HCMV+ DR7− (n=5–7) subjects determined simultaneously from the same samples per subject. E–F: Longitudinal (E) response magnitudes and (F) absolute counts of DYS+ and EPD+ CD4+ T cells from Subjects 10027 and 10069. Values in (D) represent ≥3 biological replicates with means, except for the HIV+ HCMV+ DR7− cohort with no replicates.