Productive TCRβ V and J gene pairs of bulk-sorted, inflated DYS+ CD4+ T cells are highly restricted. TCRβV and TCRβJ gene family pairings of TEM and/or TEMRA subsets of productive DYS+ and DYS− CD4 TCRs from Subjects (A) 10027, (B) 10040, (C) 10069, (D) 10032, and (E) 20. Data shown represent single experiments. V and J gene pairs are connected by stems between their arcs. Arc lengths reflect gene family proportions within the sample’s repertoire. High magnitude V and J gene families are emphasized.