FIGURE 7.

Inflated DYS⁺ CD4 T cells are CX₃CR1^high, not replicatively senescent and do not cross-react with HIV gag proteins. A–B: Ex vivo comparisons of (A) CX₃CR1 MFI and (B) Ki-67 response magnitude of DYS⁺ CD4⁺ T_EM of HIV⁺ HCMV⁺ subjects to DYS⁻ CD4⁺ T_EM of HIV⁻ HCMV⁻ controls. HIV⁺ HCMV⁺: n=7, HIV⁻ HCMV⁺: n=10 and HIV⁻ HCMV⁻: n=10. (C) CD57 and CD28 expressions of Subject 10027 DYS⁺ and DYS⁻ CD4⁺ T cells. (D) Luciferase luminescence response of 19 DR7-presented HIV gag epitopes stimulation of Subject 10027’s DYS⁺ TCR expressed in the Jurkat cell line. (E) DYS⁺ CD4 magnitude change from tp1 (HIV viremia) to tp2 (HIV aviremia). A–B, E: graphs show mean±95% CI for all subjects and represent single experiments with no replicates except (E) showing technical replicates. D: data shows mean±SD of technical duplicates.