. Author manuscript; available in PMC: 2018 Nov 1.

Published in final edited form as: J Immunol. 2017 Oct 2;199(9):3187–3201. doi: 10.4049/jimmunol.1700851

Table II.

Dominant CDR3βs of inflated DYS⁺ CD4⁺ T cells share conserved polar, neutral amino acids.

Subject ID	CDR3 freq.	TCRβV family	V segment (start of CDR3)					N1			D segment				N2	J segment								TCRβJ family
10027	94%	6-5	C	A	S			I	H	Q			G	S			T		E		A	F	F	1-1
			tgt	gcc	agc			atc	cat	caa			ggg	agc			act		gaa		gct	ttc	ttt

10040	84%	28	C	A	S				T			S	G		A	A	T	N	E	K	L	F	F	1-4
			tgt	gcc	agc				act			tca	ggg		gcg	gca	act	aat	gaa	aaa	ctg	ttt	ttt

10069	91%	6-5	C	A	S	S	Y					Q	G		A		T		E		A	F	F	1-1
			tgt	gcc	agc	agt	tat					cag	ggc		gcc		act		gaa		gct	ttc	ttt

10032	99%	6-2	C	A	S	S			F			Q	G		Q		Q			P	Q	H	F	1-5
			tgt	gcc	agc	agt			ttc			cag	ggg		caa		cag			ccc	cag	cat	ttt

20	96%	5-6	C	A	S	S	L		F		G	T	G	G		N	T		E		A	F	F	1-1
			tgt	gcc	agc	agc	ttg		ttc		ggg	aca	ggg	ggg		aac	act		gaa		gct	ttc	ttt

Conserved glutamine (Q), serine (S) and threonine (T) within the D-segment are underlined. The dominant CDR3s were identical for DYS⁺ CD4⁺ T_EM and T_EMRA in all subjects.