. Author manuscript; available in PMC: 2018 Nov 1.

Published in final edited form as: J Immunol. 2017 Oct 2;199(9):3187–3201. doi: 10.4049/jimmunol.1700851

Table III.

Dominant CDR3βs of DYS⁻ CD4⁺ T cells do not share conserved polar, neutral amino acids.

Subject	CD4 subset	CDR3 Freq.	TCRβV family	V gene start of CDR3						N1		D gene				N2		J gene							TCRβJ family
10027	T_EM	16%	12-3	C	A	S	S				P		G	T	S	A	G				E	Q	Y	F	2-7
				tgt	gcc	agc	agt				ccg		ggg	act	agc	gcc	ggc				gag	cag	tac	ttc
	T_EMRA	34%	7-9	C	A	S	S			T	L	A	G			Y		S	N	Q	P	Q	H	F	1-5
				tgt	gcc	agc	agc			acg	ttg	gcg	gga			tat		agc	aat	cag	ccc	cag	cat	ttt

10040	T_EM	7%	3-1	C	A	S	S	Q	D				G	G	S		S		S	Y	E	Q	Y	F	2-7
				tgt	gcc	agc	agc	caa	gac				ggc	ggg	agt		agc		tcc	tac	gag	cag	tac	ttc
	T_EMRA	15%	30	C	A	W				N		Q	G				R			E	P	Q	H	F	1-5
				tgt	gcc	tgg				aat		cag	ggg				cgc			gag	ccc	cag	cat	ttt

10069	T_EM	28%	9	C	A	S	S			D		R	D	R					D	Q	P	Q	H	F	1-5
				tgt	gcc	agc	agc			gac		cgg	gac	agg					gat	cag	ccc	cag	cat	ttt
	T_EMRA	28%	12-3	C	A	S	S				L		G	G		K			G	N	T	I	Y	F	1-3
				tgt	gcc	agc	agc				ctg		ggg	ggg		aaa			gga	aac	acc	ata	tat	ttt

10032	T_EM	22%	5-8	C	A	S	S	L			L	A	G					A	G	N	T	I	Y	F	1-3
				tgt	gcc	agc	agc	tta			cta	gca	ggg					gct	gga	aac	acc	ata	tat	ttt

20	T_EM	3%	20-1	C	S	A	R		D		G	P	G			I	R					A	F	F	1-1
				tgc	agt	gct	aga		gat		ggt	cca	ggg			att	cga					gct	ttc	ttt

Glutamine (Q) within the D-segment is underlined.