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. 2017 Nov 9;15:45. doi: 10.1186/s12964-017-0200-3

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — Western blot analysis demonstrating the increase of sCD146 level upon CD44 up-regulation. The expression of CD44 in (MCF7-B5-CD146) was stimulated (HA+, Dox-), the culture medium was collected at 0, 24 and 48 h. The collected media were concentrated and tested for the level of CD146 ectodomain (sCD146). Shown is the cropped blot image representing indicated protein (sCD146; ~105 kDa), and the gel has been run, for the three samples, under the same experimental conditions. The difference is considered statistically significant (student’s two-tailed t-test, ***P < 0.001)