Table 1.

Bacterial strains and plasmids used in this study

Designation	Relevant characteristics	Source/references
Plasmids
pYX68	pSC101 ori; ChlR; PrrnB; ilvE-ilvA-sdaB	[19]
pYX90	p15A ori; SpectR; P LlacO1; alsS-ilvC-ilvD-avtA	[19]
pYX97	ColE1 ori; AmpR; P LlacO1; leuDH-kivd-yqhD	[19]
pLF101	p15A ori; SpectR; P LlacO1; alsS-ilvC-ilvD	This work
pLF102	ColE1 ori; AmpR; P LlacO1; kivd-yqhD	This work
Strains
E. coli DH5α	lacZDM15 recA	NEB
E. coli YH40	BW25113/F′ [traD36, proAB +, lacIq ZΔM15] derivative with enhanced ability of amino acid utilization and with ΔglnA, ΔgdhA, ΔlsrA	[19]
E. coli AY3	E. coli YH40 with plasmids pYX68, pYX90 with the mutant genes and pYX97 with the mutant genes	[17, 19]
E. coli B	Prototroph	ATCC 11303
E. coli B01	E. coli B Δldh:: cam+	This work
E. coli BLF2	E. coli B01 with plasmids pLF101 and pLF102	This work