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. 2017 Nov 9;17:730. doi: 10.1186/s12885-017-3737-z

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Workflow of the study and characterisation of plasma EVs. a Workflow of the study. b Representative transmission electron microscopy image of plasma EVs. c Quantification of EVs isolated from plasma of PC patients and healthy controls (HC) by nanoparticle tracking analysis. d Average size distribution of EVs isolated from plasma of PC patients and healthy controls. e Western blot analysis of EV markers (TSG101, CD9), endoplasmic reticulum protein Calnexin and β-actin in plasma EVs isolated from two healthy individuals and PC-3 cells (as a positive control)