Fig. 4.

The impact of genetically deleting endothelial β4 integrin on neuroinflammation in EAE. Frozen brain sections taken from the β4-EC-KO and WT littermate control mice at the acute stage of EAE (day 21) were stained using antibodies specific for the inflammatory leukocyte markers MHC II, CD45, and Mac-1. Data points represent the mean ± SEM of events observed in the medulla oblongata (n = 4 mice). Scale bar = 100 μm. Note that quantification of all three markers revealed that β4-EC-KO brains contained increased numbers of MHC II+ and CD45+ inflammatory leukocytes, as well as increased expression level of the microglial/macrophage marker Mac-1