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. 2017 Nov 8;15:106. doi: 10.1186/s12915-017-0445-8

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© Herrmann et al. 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Mitochondria expressing AtErv1 fail to import the Mia40 substrates Tim9, Cmc1, and CCMH. a–f The indicated proteins were synthesized in the presence of [³⁵S]-methionine in reticulocyte lysate and incubated with mitochondria isolated from the indicated strains for the times indicated. Non-imported material was removed by treatment with proteinase K in all samples shown in this figure. Mitochondria were washed, re-isolated and subjected to SDS-PAGE and autoradiography; 10% of the radiolabeled protein used per time point was loaded as a control