Skip to main content
. Author manuscript; available in PMC: 2018 Oct 15.
Published in final edited form as: J Immunol. 2017 Sep 11;199(8):2767–2776. doi: 10.4049/jimmunol.1700498

Figure 5. HR+ETPs display elevated phosphorylation of STAT6 transcription factor.

Figure 5

(A) Thymic cells from IL-13Rα1-GFP reporter mice were depleted of Lin+ cells and the CD25−CD44+c-Kit+GFP+ (HR+ETPs) cells were analysed for STAT6 (pSTAT6) and ERK1/2 (pERK1/2) phosphorylation as well as expression of Egr1 and Id3 transcription factors ex vivo in comparison to CD25−CD44+c-Kit+GFP− (HR−/PETPs) counterparts. The histograms show a representative experiment and the bar graphs show compiled MFI results from 3 independent experiments. *p<0.05 and **p<0.01 as determined by a paired t-test. (B) HR+ETPs and HR−/PETPs sorted from IL-13Rα1-GFP reporter mice were analysed for Notch1 transcription ex vivo. The plot illustrates a representative experiment for Notch1 amplification while the bar graph show relative mRNA quantification (RQ) data compiled from 4 independent experiments. ***p<0.001 as determined by two-tailed, unpaired Student’s t-test.