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. Author manuscript; available in PMC: 2018 Oct 15.
Published in final edited form as: J Immunol. 2017 Sep 11;199(8):2767–2776. doi: 10.4049/jimmunol.1700498

Figure 7. In vivo inhibition of STAT6 or ERK1/2 signaling restores Notch1 expression in HR+ETPs.

Figure 7

(A) IL-13Rα1-GFP reporter mice were given i.p. STAT6 or ERK1/2 inhibitor and the Lin−CD4−CD8− thymic cells were isolated and stained with antibodies to CD25, CD44, c-Kit, pSTAT6, pERK1/2 and Id3. The histograms show a representative experiment for STAT6 and ERK1/2 phosphorylation as well as Id3 expression by CD25−CD44+ c-Kit+ HR+ (GFP+) ETPs. The bar graphs show the mean ± SD MFI results compiled from 3 independent experiments. *p<0.05 and **p<0.01 as determined by a paired t-test. (B) The HR+ETPs from (A) were also analysed for Notch1 transcription by quantitative PCR. The plot shows data from a representative Notch1 amplification experiment and the bars show relative mRNA quantification (RQ) data compiled from compiled from 2 independent experiments. **p<0.01 as determined by one way ANOVA.