Table 1.

IL-4 and IL-13 inhibit ETP T-cell lineage potential

T-cell potential (%)
Single ETP	NIL	IL-4	IL-13
HR-RV	100 (32)	18 (38)	13 (30)
Empty-RV	100 (36)	100 (31)	100 (35)

DN1a, b ETP cells from HR^−/− mice were transduced with either HR-RV or Empty-RV and single cells were sorted and cultured on OP9-DL1 stromal cells in the presence of IL-7+Flt3L alone (Nil) or with addition of IL-4 or IL-13 as described in Materials and Methods. After 10 days the cultures were analyzed for the expression of CD25 T-cell lineage marker on CD45⁺ live cells. The numbers represent the percentage of single progenitor cells that gave rise to T-lineage cells. The percentages are normalized based on the number of productive colonies (150 or more CD45⁺cells in a well) raised from single cells, excluding wells that did not support growth. The values in the parentheses represent the numbers of productive colonies. Plating efficiencies were 70 %.