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. 2017 Nov 9;14:218. doi: 10.1186/s12974-017-0982-7

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — RGC clearance and PMC appearance in axotomized retinas. Magnifications from flat-mounted traced retinas analyzed at 2 (a, b), 5 (c, d), 9 (e, f), 14 days (g, h), or 2 months (i, j) after optic nerve crush (first column) or transection (second column) showing the course of FG⁺RGC disappearance and the appearance of PMCs (yellow arrows). k–n Images from the central dorso-temporal area (drawing in k, top right) of a representative retina analyzed 14 days after ONT and immunodetected with Iba1 and Brn3a. PMCs are those MCs transcellularly labeled (FG⁺Iba⁺). In these injured retinas, majority of FG⁻Iba⁺ MCs are probably activated, but have not yet engulfed a traced RGC