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The CuAAC reaction of the peptide AP (50 µM) with propargyl alcohol (50 µM), ligand (100 µM), CuSO4 (50 µM), and sodium ascorbate (500 µM) in Milli-Q water at 24 ± 1 °C in air to form the product TP and the oxidation byproducts (2-imidazolidone derivatives) API/II/III, TPI/II/III as well as a complex mixture of other oxidation byproducts.
