Figure 1. Nervous system Mettl14 deletion results in residual radial glia cells and ongoing neurogenesis in the postnatal mouse cortex.

(A–C) Presence of neurogenic RGCs in P5 Nestin-Cre;Mettl14^f/f cKO cortices. Shown are sample confocal images (A, B) and quantifications (C). Regions in white boxes are shown at a higher magnification. Scale bars: 500 μm (A, top panel), 50 μm (A, bottom panel), 100 μm (B). Values in (C) represent mean ± SEM (n = 4–7; ***: P < 0.001; *: P < 0.05; unpaired Student’s t-test).

(D–E) Presence of IPCs in P5 cKO cortices. Shown are sample confocal images (D; scale bars: 100 μm) and quantification (E). Values represent mean ± SEM (n = 6; ***: P < 0.001; **: P < 0.01; unpaired Student’s t-test).

(F–G) Ongoing neurogenesis in P5 cKO cortices. P5 pups were injected with EdU and analyzed 48 hr later. Shown in (F) are sample confocal images of the ventricular side of the primary somatosensory cortex. Arrows indicate Pax6⁺EdU⁺ cells (top) and Tbr2⁺TuJ1⁺EdU⁺ cells (bottom). Scale bars: 100 μm. Quantification of EdU⁺ cells with different markers is shown in (G). Values represent mean ± SEM (n = 6; ***: P < 0.001; unpaired Student’s t-test).

(H–K) Reduced production of upper-layer neurons and astrocytes in cKO cortices. Pregnant mice were injected with EdU at E15.5 and analyzed at P5. Shown are sample confocal images (H, J; scale bars: 100 μm) and quantification (I, K). Values represent mean ± SEM (n = 6; ***: P < 0.001; unpaired Student’s t-test).

See also Figure S1