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. Author manuscript; available in PMC: 2018 Nov 2.
Published in final edited form as: Cell. 2017 Sep 28;171(4):877–889.e17. doi: 10.1016/j.cell.2017.09.003

Figure 6. METTL14 regulates cell cycle progression of human NPCs.

Figure 6

(A–B) Flow cytometry analysis of cell cycle progression of hNPCs with METTL14 KD. Human NPCs were electroporated to co-express GFP and shRNA-control, or shRNA-METTL14. After 4 days, hNPCs were pulse-labeled with EdU (10 μM) for 30 min, incubated for 14 hr, followed by EdU and DNA content (DyeCycle Violet) staining and flow cytometry analysis, similarly as in Figure 3C–D. Values represent mean ± SEM (n = 4; **: P < 0.01; unpaired Student’s t-test).

(C–D) Flow cytometry analysis of cell cycle progression with METTL14 KD in human forebrain organoids. Day 45 forebrain organoids were electroporated to co-express GFP and shRNA-control, or shRNA-METTL14. After 7 days, forebrain organoids were pulse-labeled with EdU (10 μM) for 1 hr, cultured further for 14 hr, followed by dissociation and analysis similarly as in Figure 3C–D. Values represent mean ± SEM (n = 4; ***: P < 0.001; unpaired Student’s t-test).

See also Figure S6.