Figure 1.

Time evolution of TIRFM signals from a 10ms single Ca²⁺-channel opening. The distance in the positive x-direction was varied to show how ΔF/F_o is dependent upon distance from the channel. The top and bottom insets show the peak ΔF/F_o and time to equilibrate (Time to Eq.) after the channel has closed as a function of distance from the channel. Using standard conditions: D_Ca=223µm²/s, D_CaF=15µm²/s, α_f=150 µM⁻¹s⁻¹, and β_f=450 s⁻¹.