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The effect of diffusion coefficients, depth of the channel, on- and off-rates of Ca2+ binding to the dye on peak fluorescence. Maximum ΔF/Fo as a function of depth as αf increases due to increasing rc with (A) DCa=200µm2/s, (B) DCa=300µm2/s, (C) DCa=600µm2/s, and (D) DCa=700µm2/s.
