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Mobile buffer EGTA does not affect the kinetics of TIRFM signals. Time evolution of TIRFM signals from a 10ms single Ca2+-channel opening with increasing [EGTA]=0 (black),100 (blue), 1000µM (red) for A) DCa=600µm2/s, αf=600/(µMs), depth=250nm, B) DCa=700µm2/s, αf=700/(µMs), depth=200nm, and C) DCa=700µm2/s, αf=1400/(µMs), depth=250nm.
