Simulating two channels with optimal conditions. Time trace simulation of 2 channels in which the second channel located 100nm away from the center channel opens 2ms after the first channel closes with (A) DCa=600µm2/s, αf=150/(µMs), depth=250nm, (B) DCa=700µm2/s, αf=700/(µMs), depth=200nm, and (C) DCa=700µm2/s, αf=1400/(µMs), depth=250nm