Table 1.

Parameter values used for simulations

Parameters	Values
Height of cytosol box	2.05µM
Length of cytosol box	4.05 µM
Width of cytosol box	4.05 µM
Free Ca2+ diffusion coefficient (Dca)	200–700 µm2s−1
Resting Ca2+ concentration ([Ca2+]rest)	0.05 µM
Bound indicator dye and Ca2+ diffusion coefficient (DCaF)	Dca/3
Total indicator dye concentration ([Cal]T)	40µM
Dissociation constant (κF)	0.3 µM
Smoluchowski capture radius (rc)	0.00005–0.0008µm
On-rate (αf)	see eq. 3 µM−1s−1
Off-rate (βf)	κF*αf
Mobile Buffer (EGTA) diffusion Coefficient (DCaM)	200 µm2s−1
Total mobile buffer concentration ([EGTA])	0µM – 1000µM
Dissociation constant (κM)	0.15 µM
On-rate (αm)	5 µM−1s−1
Off-rate (βm)	0.75 s−1
Total stationary buffer concentration ([S]T)	300 µM
Dissociation constant (κT)	2 µM
On-rate (αs)	400 µM−1s−1
Off-rate (βs)	800 µM−1s−1
Maximum Ca2+ current (IMax)	0.1 pA
Endoplasmic Reticulum concentration ([Ca2+]Er)	600 µM