| DNA | 20 ng (or 5 μl of cell lysate [see annotation]) |
| dNTP (10mM) | 1 μl |
| Forward Primer 1 (10uM) | 1 μl |
| Reverse Primer 1 (10uM) | 1 μl |
| 5X Q5 Reaction Buffer | 10 μl |
| Q5 HF DNA Polymerase | 0.5 μl |
| Nuclease-Free Water | to 50 μl |
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