| Template DNA | 40 ng (or 1 μl of PCR product, treated with ExoSap according to the manufacturer’s instructions) |
| dNTP (10mM) | 1 μl |
| Primer F_2 (10 μM) | 1 μl |
| Primer R_2 (10 μM) | 1 μl |
| 5X Q5 Reaction Buffer | 10 μl |
| Q5 HF DNA Polymerase | 0.5 μl |
| Nuclease-Free Water | to 50 μl |
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