Current Protocols in Molecular Biology: Mapping Transposon Insertions in Bacterial Genomes by Arbitrarily Primed PCR

. Author manuscript; available in PMC: 2018 Apr 3.

Published in final edited form as: Curr Protoc Mol Biol. 2017 Apr 3;118:15.15.1–15.15.15. doi: 10.1002/cpmb.38

Initial denaturation:	Denature: 1 min 95°C
30 cycles:	Denature: 30 sec 95°C
	Anneal: 30 sec X°C^a
	Extend: 120 sec 72°C
Final extension:	Extend: 4 min 72°C
	Hold: ∞ 12°C
The denaturation and extension temperatures are specific to the polymerase chosen. ^aThe annealing temperature is specific to the 2_F primer chosen (Table 1).