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. Author manuscript; available in PMC: 2018 Dec 1.
Published in final edited form as: Hum Mutat. 2017 Sep 23;38(12):1774–1785. doi: 10.1002/humu.23339

Figure 3. DEAF1 variants have altered DNA binding.

Figure 3

Electrophoretic mobility shift assays (EMSA) showed decreased DNA binding affinity of the four novel DEAF1 variants to the 11-space and 6-space double stranded DNA DEAF1 binding sequences [Vulto-van Silfhout et al., 2014], compared to WT DEAF1. The DEAF1 ADWA variant, which has previously been shown to be defective in binding DNA [Bottomley et al., 2001], was used as a negative control.