(A) DIO Klbfl/fl and KlbAlb littermates were i.p. injected with 1 mg/kg FGF19, FGF21 or vehicle and sacrificed 6 hr later. Cyp7a1 mRNA in liver was measured by QPCR. n = 5/group.
(B and C) DIO Klbfl/fl and KlbAlb littermates were i.p. injected with 1 mg/kg FGF19, FGF21 or vehicle and sacrificed 30 min later. (B) Phosphorylated ERK1/2 normalized to total ERK1/2 protein in liver was measured by western blot. (C) Egr1 mRNA levels in liver was measured by QPCR. n = 6/group. QPCR cycle time values are shown for the vehicle-treated Klbfl/fl group.
Data are shown as the mean ± SEM. **p < 0.01, ***p<0.001 compared to control. N.S., not significant. Significant interactions between genotype (Klbfl/fl vs. KlbAlb) and treatment (Vehicle vs. FGF19) were detected for p-ERK1/2/T-ERK/1/2 (p=0.0007) and Egr1 mRNA (p=0.004).
See also Figure S1 and Table S1.