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Comparison of the sensitivity of simultaneously detecting the targeting genes Z3276, stx1, and stx2 in E. coli O157:H7 by the multiplex real-time PCR assay. The standard curves of Z3276, stx1, and stx2 created by the multiplex real-time PCR assay (a); the standard curves of Z3276, stx1, and stx2 generated by three individual simplex real-time PCR assays (b). The C T values represent the average of six replicates from two independent tests ±SD
