Table 2.

Detection results of the exclusivity test with different bacterial strains by the multiplex real-time PCR assay

				Target gene
Genus	Species	Pathotype	Strain name/Serotype	Z3276	stx1	stx2
Escherichia	coli	EHEC	EDL933/O157:H7	+	+	+
Escherichia	coli	ETEC	EC1775/O26:H11	_	+	_
Escherichia	coli	STEC	EC1803/O103:H11	_	+	+
Escherichia	coli	STEC	EC1807/O121:H19	_	_	+
Escherichia	coli	ETEC	EC1801	_	+	_
Escherichia	coli	EPEC	EC1501	_	_	_
Escherichia	coli	EIEC	EC1513	_	_	_
Escherichia	coli	EDC	DEC5A	_	_	_
Escherichia	coli		K12/MG1655	_	_	_
				_	_	_
Salmonella	enterica		SL192/Typhi	_	_	_
Salmonella	enterica		SL317/Newport	_	_	_
Salmonella	enterica		SL535/Typhimurim	_	_	_
				_	_	_
Shigella	sonnei		SH20145	_	_	_
Shigella	dysenteriae		SH20152	_	_	_
Shigella	flexneri		SH20155	_	_	_
Shigella	boydii		SH20140	_	_	_
				_	_	_
Staphylococcus	aureus		ATCC25923	_	_	_
Staphylococcus	epidermidis		ATCC12228	_	_	_
Staphylococcus	pyogenes		ATCC19615	_	_	_
				_	_	_
Vibrio	alginolytica		ATCC17749	_	_	_
Vibrio	parahemolyticus		ATCC17802	_	_	_
Vibrio	vulasfians		ATCC27562	_	_	_
				_	_	_
Enterobacter	cloacae		ATTCC23355	_	_	_
Enterobacter	cloacae		ATCC13047	_	_	_
Enterobacter	cloacae		ATCC13048	_	_	_
				_	_	_
Citrobacter	freundii		ATCC8090	_	_	_
				_	_	_
Klebsiella	pneumoniae		ATCC13883	_	_	_
				_	_	_
Pseudomonas	aeruginosa		ATCC27853	_	_	_