Table 3.
Primers and probes used in the multiplex real-time PCR assay
| Target gene | Primer/Probe | Sequence (5' -- 3') | Amplicon | Reference |
|---|---|---|---|---|
| length (bp) | ||||
| Z3276 | Z3276 forward | TATTCCGCGATGCTTGTTTTT | 130 | Li and Chen. 2012 |
| Z3276 reverse | ATTATCTCACCAGCAAACTGGCGG | |||
| Z3276 probe | FAM-CCCGCAAATCTTTCCMGBNFQ | |||
| stx1 | stx1 forward | GGATTTCGTACAACACTGGATGAT | 67 | This study |
| stx1 reverse | ATCCACATCTTCAGCAGTCATTACA | |||
| stx1 probe | TAMRA-CAGTGGGCGTTCTTMGBNFQ | |||
| stx2 | stx2 forward | GGGCAGTTATTTTGCTGTGGAT | 59 | This study |
| stx2 reverse | GGTCAAAACGCGCCTGAT | |||
| stx2 probe | JOE-ACGAGGGCTTGATGTCMGBNFQ | |||
| IAC | IAC forward | CAGGATTAGCAGAGCGAGGTATG | 65 | Fricker et al. 2007 |
| IAC reverse | CGTAGTTAGGCCACCACTTCAAG | |||
| IAC probe | CY5-AGGCGGTGCTACAGAG-MGBNFQ |