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. 2017 Nov 9;12(11):e0187436. doi: 10.1371/journal.pone.0187436

Fig 2. Workflow for the iTRAQ-based quantitative proteomic experiment.

Fig 2

Protein samples were obtained from vernalization-treated (T) and non-treated (CK) faba bean seedlings. Three biological replicates of T and CK samples were digested and labeled with iTRAQ tags, followed by the SCX fractionation. The sufficient amounts of labeled peptides were used for LC-MS/MS analysis.