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. 2017 Oct 30;13(10):e1006700. doi: 10.1371/journal.ppat.1006700

Fig 7. Deletion of Salmonella effector SifA abrogates Vps41 recruitment to SCV membranes.

Fig 7

a-f) HeLa cells were infected with either DsRed expressing-wild-type (WT) strain of Salmonella (NCTC 12023 in (a) and SL1344 in (c)) or sifA sseJ, sseF, sseG, and pipB2 strains, followed by transfection with HA tagged-Vps41. Cells were fixed at 10 hr p.i., and co-stained with anti-HA (green) and anti-LAMP1 (blue) antibodies. Different panels represent a higher magnification of the boxed areas. Bars: (main) 10 μm; (insets) 5 μm. g and h) Quantification of Vps41-positive SCVs in HeLa cells infected with different Salmonella strains (as labeled) and fixed at 10 hr p.i. Data represent mean ± S.D. from three independent experiments where ~100 SCVs were counted in each experiment (n.s., not significant; ****, P < 0.0001; Student’s t test).