|
Salmonella typhimurium strains: |
|
S. typhimurium SL1344 |
Wild-type (WT); Streptomycin 50 μg/ml |
Kind gift from Dr. John Brumell and described previously [62]. |
| GFP-S. typhimurium SL1344 |
S. typhimurium SL1344 transformed with GFP expressing plasmid pFU95; Streptomycin 50 μg/ml Ampicillin 50 μg/ml |
This Study |
| DsRed-S. typhimurium SL1344 |
S. typhimurium SL1344 transformed with DsRed expressing plasmid pFU96; Streptomycin 50 μg/ml Ampicillin 50 μg/ml |
This Study |
|
S. typhimurium SL1344 ΔsifA
|
S. typhimurium SL1344 with chromosomal deletion of SifA gene; Streptomycin 50 μg/ml |
Kind gift from Dr. John Brumell and described previously [62]. |
|
S. typhimurium SL1344 ΔsifA/psifA-2HA |
S. typhimurium SL1344 with chromosomal deletion of SifA gene complemented with a plasmid encoding epitope tagged SifA; Chloramphenicol 34 μg/ml |
Kind gift from Dr. John Brumell and described previously [62]. |
|
S. typhimurium SL1344 ΔsifA/psifA (L130D)-2HA |
S. typhimurium SL1344 with chromosomal deletion of SifA gene complemented with a plasmid encoding epitope tagged SifA having point mutation at amino acid residue position 130 (L to D); Streptomycin 50 μg/ml Chloramphenicol 34 μg/ml |
This study |
|
S. typhimurium NCTC 12023 |
Wild-type (WT) |
Kind gift from Dr. Michael Hensel and described previously [15]. |
| DsRed-S. typhimurium NCTC 12023 |
S. typhimurium NCTC 12023 transformed with DsRed expressing plasmid pFU96; Ampicillin 50 μg/ml |
This Study |
|
S. typhimurium NCTC 12023 (ΔsifA::FRT ΔsseJ::FRT) |
S. typhimurium NCTC 12023 with a chromosomal deletion of effectors SifA and SseJ |
Kind gift from Dr. Michael Hensel and described previously [15]. |
|
S. typhimurium NCTC 12023 (ΔproC::FRT) |
S. typhimurium NCTC 12023 with a chromosomal deletion of Pyrroline-5-carboxylate reductase gene |
Kind gift from Dr. Michael Hensel and described previously [15]. |
|
S. typhimurium SL1344 (ΔsseF::aphT) |
S. typhimurium SL1344 with a chromosomal deletion of effector SseF; Streptomycin 30 μg/ml Kanamycin 50 μg/ml |
Kind gift from Dr. Michael Hensel. |
|
S. typhimurium SL1344 ΔsseF/ProsseAsscBsseF::HA |
S. typhimurium SL1344 with chromosomal deletion of SseF gene complemented with a plasmid encoding HA-epitope tagged SseF; Streptomycin 30 μg/ml Kanamycin 50 μg/ml Carbenicillin 50 μg/ml |
This study |
|
S. typhimurium SL1344 (ΔsseG::aphT) |
S. typhimurium SL1344 with a chromosomal deletion of effector SseG; Streptomycin 30 μg/ml Kanamycin 50 μg/ml |
Kind gift from Dr. Michael Hensel. |
|
S. typhimurium SL1344 (ΔpipB2::aphT) |
S. typhimurium SL1344 with a chromosomal deletion of effector PipB2; Streptomycin 30 μg/ml Kanamycin 50 μg/ml |
Kind gift from Dr. Michael Hensel. |
|
Yeast two-hybrid constructs: |
| pGADT7 vector |
GAL4-activation domain encoding yeast two-hybrid vector |
Clontech |
| pGADT7-Vps39 |
Human Vps39 cloned into the pGADT7 vector |
Described previously [27]. |
| pGADT7-PLEKHM1 |
Human PLEKHM1 cloned into the pGADT7 vector |
Described previously [26]. |
| pGADT7-SKIP |
Human SKIP cloned into the pGADT7 vector |
Described previously [27]. |
| pGBKT7 vector |
GAL4-DNA binding domain encoding yeast two-hybrid vector |
Clontech |
| pGBKT7-SifA |
SifA cloned into the pGBKT7 vector |
This study |
|
Yeast three-hybrid constructs: |
| pBridge vector |
Yeast three-hybrid vector |
Clontech |
| pBridge-SifA |
SifA (1–330 aa) cloned into the MCS-I of the pBridge vector |
This study |
| pBridge-SifA/SKIP |
SifA (1–330 aa) cloned into the MCS-I and full-length SKIP cloned into the MCS-II of the pBridge vector |
This study |
| pBridge-SifA/SKIP (G828D) |
SifA (1–330 aa) cloned into the MCS-I and SKIP with point mutation at amino acid position 828 changing G with D, cloned into the MCS-II of the pBridge vector |
This study |
| pBridge-SifA/PLEKHM1 |
SifA (1–330 aa) cloned into the MCS-I and full-length PLEKHM1 cloned into the MCS-II of the pBridge vector |
This study |
|
Mammalian expression constructs: |
| pcDNA3.1(-) vector |
Mammalian expression vector |
Invitrogen |
| pcDNA3.1(-)- FLAG- TGFBRAP1 |
N-terminal FLAG-tagged human TGFBRAP1 cloned into the pcDNA3.1(-) vector |
This study |
| pcDNA3.1(-)- FLAG- PLEKHM1 |
N-terminal FLAG-tagged human PLEKHM1 cloned into the pcDNA3.1(-) vector |
Described previously [26]. |
| pEGFPC1-PLEKHM1 |
N-terminal GFP-tagged human PLEKHM1 cloned into the pEGFPC1 vector |
Described previously [26]. |
| pcDNA3.1(-)-FLAG-SKIP |
N-terminal FLAG-tagged human SKIP cloned into the pcDNA3.1(-) vector |
Described previously [26]. |
| pcDNA3.1(-)-siRNA-resistant-FLAG-SKIP |
N-terminal FLAG-tagged full-length rescue construct against SKIP-siRNA cloned into the pcDNA 3.1(-) vector |
Described previously [27]. |
| pEGFPC1-SKIP |
N-terminal GFP-tagged human SKIP cloned into the pEGFPC1 vector |
Described previously [26]. |
| pcDNA3.1(+)-Arl8b untagged |
Human Arl8b cloned into the pcDNA3.1(+) vector |
Described previously [26]. |
| pcDNA3.1(+)-Mouse Arl8b-GFP |
Full-length mouse Arl8b with C-terminal GFP tag cloned into the pcDNA3.1(+) vector |
Described previously [26]. |
| pEGFPC1-Rab7 |
N-terminal GFP-tagged human Rab7 cloned into the pEGFPC1 vector |
Kind gift from Dr. Vesa Olkkonen and described previously [75]. |
| pEBB-HA-Rab7 |
Full-length human Rab7 with N-terminal HA tag cloned into the pEBB vector |
Kind gift from Dr. Jason Kinchen and described previously [76]. |
| pcDNA3.1(-)-HA-Vps41 |
N-terminal HA tagged human Vps41 cloned into the pcDNA3.1(-) vector |
Described previously [27]. |
| pEGFPC1-Vps41 |
N-terminal GFP-tagged human Vps41 cloned into the pEGFPC1 vector |
This study |
| pcDNA3.1(-)-HA-Vps39 |
N-terminal HA tagged human Vps39 cloned into the pcDNA3.1(-) vector |
Described previously [27]. |
| pcDNA3.1(-)-Vps33a-HA |
C-terminal HA tagged human Vps33a cloned into the pcDNA3.1(-) vector |
Described previously [27]. |
| GFP-LAMP1 |
GFP-tagged Lgp120 (rat lamp-1) |
Kind gift from Dr. Steve Caplan and described previously [44]. |
| pEGFPC1-SifA |
SifA cloned into the pEGFPC1 vector |
Kind gift from Dr. Samuel Miller and described previously [77]. |
| Myc-SifA |
SifA cloned into the pCMV-myc vector |
Kind gift from Dr. Samuel Miller and described previously [21]. |
| Myc-SifA (L130D) |
Myc-SifA construct with point mutation at amino acid residue position 130 (L to D) of SifA |
This Study |
| Myc-SifA (M131D) |
Myc-SifA construct with point mutation at amino acid residue position 131 (M to D) of SifA |
This Study |
|
Bacterial expression constructs: |
| pGEX-5X-1-SifA |
SifA cloned in pGEX-5-X-1 vector |
Kind gift from Dr. Kasturi Haldar and described previously [78]. |
| pFU95 |
Apr; gapA-rbs-gfpmut3.1; ColE1 |
Kind gift from Dr. Petra Dersch and described previously [79]. |
| pFU96 |
Apr; gapA-rbs-dsred2; ColE1 |
Kind gift from Dr. Petra Dersch and described previously [79]. |
| pSifA-2HA |
2 HA-tags SifA cloned into the pACYC184 vector |
Kind gift from Dr. John Brumell and described previously [62]. |
| pSifA (L130D)-2HA |
psifA-2HA construct with a point mutation at amino acid residue 130 (L to D) of SifA |
This Study |
| ProsseAsscBsseF::HA |
pWSK29 plasmid containing expression cassette for HA epitope-tagged SseF effector |
Kind gift from Dr. Michael Hensel and described previously [80]. |
