Fig 1. AtHKT1 contributes to salt tolerance in a linear manner.

(A) Flowers and siliques of Tsu-1, Col-0, Col-0 AtHKT1 RNAi lines, and athkt1 under normal conditions (upper) or treated with 100 mM NaCl (lower) for two weeks. (B-C) Numbers of seeds from the plants shown in (A) under normal (B) and 100 mM NaCl treatment conditions (C). Two-week-old plants were treated without or with twice 100 mM NaCl, and seeds were collected continuously during silique maturation. (D) AtHKT1 expression level assessed by qRT-PCR in the roots of 4-week-old Tsu-1, Col-0, Col-0 AtHKT1 RNAi lines, and athkt1 grown in liquid culture. (E-F) Correlation of seed number and AtHKT1 expression level under normal (E) and salt stress conditions (F). Data represented with mean ± SE, n = 12, significant differences were determined by ANOVA.