Fig 4. Reciprocal grafting experiments revealed that the salt tolerance phenotype of Tsu-1 is driven by shoot AtHKT1, whereas the phenotype of Col-0 is driven by root AtHKT1.

(A) Plants generated via reciprocal graft between Col-0 and athkt1 treated with 100 mM NaCl for 2 weeks. (B) Numbers of seeds from reciprocally grafted Col-0 and athkt1 plants treated with 100 mM NaCl. (C) Reciprocally grafted plants between Tsu-1 and hkt1-c1subjected to 100 mM NaCl for 2 weeks. (D) Numbers of seeds from reciprocally grafted Tsu-1 and hkt1-c1 plants subjected to 100 mM NaCl. Successfully grafted plants (n > 12) were transferred to pots for 2 weeks following 100 mM NaCl treatment twice for 2 weeks before images were taken. Data represented with mean ± SE; significant differences were determined by ANOVA.