Fig 5. Substitutions in Ssa1 allow growth of sis1-Δ cells.
(A,B) Ten-fold serial dilutions of sis1-Δ strains harboring a plasmid with an insert of a WT SIS1 gene (SIS1), the originally isolated SSA1 suppressor mutant (ssa1SUP) or the indicated SSA1 substitutions. Plates were incubated at indicated temperatures for 4 days. (C) sis1-Δ cells harboring a plasmid with a WT SIS1 gene and URA3 marker plus the indicated additional TRP1-marked plasmid were streaked onto plates containing 5-FOA and incubated for 5 days at 30°C. TRP1 plasmids had inserts encoding WT (SIS1), or SSA1 genes, either WT (SSA1) or indicated variants. ΔEEVD indicates that the C-terminal four residues are absent.