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. 2017 Oct 30;13(10):e1006713. doi: 10.1371/journal.ppat.1006713

Fig 4. EAP30 silencing decreases poly(I:C)- and SeV-induced ISGs expression but not ISG induction by IFN-α.

Fig 4

(A) qPCR analysis of OASL, PKR, MX1 and ISG15 mRNA levels in PH5CH8 cells transfected with control, IRF3, or EAP30 siRNA for 48 h, and mock-treated (empty bars)or stimulated with poly(I:C) (10 μg/ml) for additional 8 h (gray bars). (B) Immunoblotting of ISG56, IRF3, EAP30 and actin proteins under experimental conditions of (A). (C) qPCR quantification of OASL, PKR, MX1 and ISG15 mRNA levels in PH5CH8 cells transfected with control, IRF3, or EAP30 siRNA for 48 h, and mock-treated (empty bars)or infected with SeV (160 HAU/ml) for additional 8 h (solid bars). (D) Immunoblotting of ISG56, SeV, IRF3, EAP30 and actin proteins under experimental conditions of (C). (E) qPCR quantification of OASL, PKR, MX1 and ISG15 mRNA levels in PH5CH8 cells transfected with control, IRF3, or EAP30 siRNA for 48 h, and mock-treated (empty bars)or stimulated with IFN-α (100 U/ml) for additional 8 h (solid bars). “*” and “**” denote statistical differences exist as compared with control siRNA-transfected cells with a P-value of < 0.05 and < 0.01, respectively.