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. 2017 Oct 30;13(10):e1006713. doi: 10.1371/journal.ppat.1006713

Fig 7. EAP30 forms a complex with IRF3 and CBP.

Fig 7

HEK293FT cells were cotransfected with empty vector, HA-EAP30 or HA-EAP20, respectively, along with a FLAG-CBP-HA construct for 48 h, followed by infection with SeV for additional 8 h. Nuclear extracts (NE) were isolated and used for immunoblotting (lanes 1–6) and co-IP analysis (lanes 7–18). Immunoprecipitation was performed by using anti-CBP pAb (lanes 7–12) or anti-IRF3 pAb (lanes 13–18), followed by immunoblotting with anti-CBP, anti-IRF3, and anti-HA antibodies. Empty circles shown on the left to the IRF3 immunoblot denote non-specific bands that served as a loading control.