Fig 5. Titration of the DinGNm DNA unwinding activity.
DNA unwinding activity was tested on 1 nM forked DNA substrate (T1+B1 oligo dimer with a 30mer complementary region and 30mer tails) with increasing concentrations of DinGNm or DinGNmK72A. A) A representative gel showing educts and unwinding reaction products, also schematic depicted on the right. Lanes: 1) heat-denatured substrate, 2) no enzyme, 3–8) 50 nM, 100 nM, 200 nM, 400 nM, 800 nM, and 1600 nM DinGNm, respectively, 9–14) 50 nM, 100 nM, 200 nM, 400 nM, 800 nM, and 1600 nM DinGNmK72A, respectively. B) Quantitation of the unwinding activity of DinGNm and DinGNmK72A. The average of three independent experiments and standard deviations are shown.