Table 1.
Strain | InhA | Number of isolates | Solid medium MICA | Liquid medium IC90 (fold shift vs WT)B | ||||
---|---|---|---|---|---|---|---|---|
NITD-916 | INH | ETH | TRI | NITD-916 | INH | |||
| ||||||||
H37Rv | WT | 0.08 μM | 0.8 μM | 6.3 μM | 50 μM | 0.1 μM | 1 μM | |
LP-0532543-RM18 | S19W | 1 | 8x | 4x | - | - | 12x | - |
LP-0532543 RM28 | I21M | 1 | 8x | 4x | - | - | 4x | - |
LP-0532543-RM34 | I21V | 1 | 4x | 4x | - | - | 3x | 2x |
LP-0532543-RM13 | F41L | 1 | 8x | - | - | - | 13x | - |
LP-0532543-RM19 | I47L | 1 | 8x | - | - | - | 7x | - |
LP-0532543-RM1 | S94A | 2 | 8x | 8x | 8x | - | 10x | 2x |
LP-0532543-RM6 | M103T | 2 | 8x | - | - | - | 10x | - |
LP-0532543-RM2 | D148E | 1 | 8x | - | - | - | >10x | - |
LP-0532543-RM4 | M161L | 3 | 8x | - | - | - | >28x | - |
LP-0532543-RM41 | R195G | 1 | 8x | 8x | - | - | >10x | 2x |
LP-0532543-RM9 | I202F | 1 | 8x | - | - | - | 9x | - |
LP-0532543-RM7 | G205A | 2 | 8x | - | - | - | 12x | - |
LP-0532543-RM11 | G205S | 1 | 8x | - | - | - | 13x | - |
LP-0532543-RM3 | A206E | 4 | 8x | - | - | - | 19x | - |
LP-0532543-RM44 | 210 | 1 | 8x | 8x | - | - | ND | ND |
LP-0532543-RM14 | G212D | 1 | 8x | - | - | - | 24x | - |
LP-0532543-RM5 | Q214P | 1 | 8x | 8x | - | - | ND | ND |
LP-0532543-RM16 | I215S | 1 | 8x | - | - | - | 9x | - |
LP-0532543-RM37 | L269R C-15T | 6 | 8x | 8x | - | - | 11x | - |
LP-0532543-RM30 | upstream of fabG1inhA | 1 | 8x | 4x | 4x | - | 6x | 5x |
MIC for WT was determined on solid medium. For resistant isolates, strains were plated onto 4x and 8x MIC for each compound on solid medium and growth scored.
IC90 was determined in liquid medium. For resistant isolates, IC90, data are presented as the fold-change compared to WT.
- denotes no shift
ND - not determined
INH - isoniazid ETH –Ethionamide TRI - Triclosan