Table 1.
Genotypes and phenotypes of M. tuberculosis H37Rv mutants resistant to NITD-916
| Strain | InhA | Number of isolates | Solid medium MICA | Liquid medium IC90 (fold shift vs WT)B | ||||
|---|---|---|---|---|---|---|---|---|
| NITD-916 | INH | ETH | TRI | NITD-916 | INH | |||
|
| ||||||||
| H37Rv | WT | 0.08 μM | 0.8 μM | 6.3 μM | 50 μM | 0.1 μM | 1 μM | |
| LP-0532543-RM18 | S19W | 1 | 8x | 4x | - | - | 12x | - |
| LP-0532543 RM28 | I21M | 1 | 8x | 4x | - | - | 4x | - |
| LP-0532543-RM34 | I21V | 1 | 4x | 4x | - | - | 3x | 2x |
| LP-0532543-RM13 | F41L | 1 | 8x | - | - | - | 13x | - |
| LP-0532543-RM19 | I47L | 1 | 8x | - | - | - | 7x | - |
| LP-0532543-RM1 | S94A | 2 | 8x | 8x | 8x | - | 10x | 2x |
| LP-0532543-RM6 | M103T | 2 | 8x | - | - | - | 10x | - |
| LP-0532543-RM2 | D148E | 1 | 8x | - | - | - | >10x | - |
| LP-0532543-RM4 | M161L | 3 | 8x | - | - | - | >28x | - |
| LP-0532543-RM41 | R195G | 1 | 8x | 8x | - | - | >10x | 2x |
| LP-0532543-RM9 | I202F | 1 | 8x | - | - | - | 9x | - |
| LP-0532543-RM7 | G205A | 2 | 8x | - | - | - | 12x | - |
| LP-0532543-RM11 | G205S | 1 | 8x | - | - | - | 13x | - |
| LP-0532543-RM3 | A206E | 4 | 8x | - | - | - | 19x | - |
| LP-0532543-RM44 | 210 | 1 | 8x | 8x | - | - | ND | ND |
| LP-0532543-RM14 | G212D | 1 | 8x | - | - | - | 24x | - |
| LP-0532543-RM5 | Q214P | 1 | 8x | 8x | - | - | ND | ND |
| LP-0532543-RM16 | I215S | 1 | 8x | - | - | - | 9x | - |
| LP-0532543-RM37 | L269R C-15T | 6 | 8x | 8x | - | - | 11x | - |
| LP-0532543-RM30 | upstream of fabG1inhA | 1 | 8x | 4x | 4x | - | 6x | 5x |
A
MIC for WT was determined on solid medium. For resistant isolates, strains were plated onto 4x and 8x MIC for each compound on solid medium and growth scored.
B
IC90 was determined in liquid medium. For resistant isolates, IC90, data are presented as the fold-change compared to WT.
- denotes no shift
ND - not determined
INH - isoniazid ETH –Ethionamide TRI - Triclosan