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. Author manuscript; available in PMC: 2017 Nov 9.
Published in final edited form as: Nat Cell Biol. 2016 Nov 21;18(12):1324–1335. doi: 10.1038/ncb3441

Figure 2.

Figure 2

PIPKIα and PI(3)K interact on the WW and IQ domains of IQGAP1. (a,b) PIPKIα and the p85 subunit of PI(3)K-binding sites on IQGAP1 were mapped with the indicated truncation mutants (Supplementary Fig. 2a) by immunoprecipitation. (c,d) In vitro binding experiments with the indicated recombinant proteins, representative of at least three experiments. (e) IQGAP1 binding sites were mapped in vitro with the indicated bovine p85α fragments. (f) A model summary of in vitro binding experiments. Unprocessed original scans of blots are shown in Supplementary Fig. 7.