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. Author manuscript; available in PMC: 2017 Nov 9.
Published in final edited form as: Nat Cell Biol. 2016 Nov 21;18(12):1324–1335. doi: 10.1038/ncb3441

Figure 3.

Figure 3

IQGAP1 physically links PIPKIα to PI(3)K. (a) The indicated endogenous proteins were immunoprecipitated from Hs578T cell lysates. Associated proteins were analysed by immunoblotting. (b–d) Hs578T cells were transfected with non-targeting (siCon), IQGAP1, p110α or PIPKIα siRNA for 48 h. The indicated proteins were immunoprecipitated and associated molecules were analysed by immunoblotting. (e) Hs578T cells were transfected with empty vector (Mock) or Myc-IQGAP1 for 48 h. p110α was immunoprecipitated and associated PIPKIα was analysed by immunoblotting. (f) Immunoblots for e were quantified and the graph is shown as mean ± s.d. of n=3 independent experiments. Rel., relative. **P = 0.028. Unprocessed original scans of blots are shown in Supplementary Fig. 7.