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. Author manuscript; available in PMC: 2018 Nov 15.
Published in final edited form as: J Immunol. 2017 Oct 6;199(10):3525–3534. doi: 10.4049/jimmunol.1701011

FIGURE 2.

FIGURE 2

IFN-β drives the type I IFN profile in B6.C3-Bbaa1 BMDMs stimulated with B. burgdorferi. (A) qRT-PCR analysis of transcripts after 6 h stimulation with live B. burgdorferi (10:1 MOI) and treatment with anti-IFNAR1, anti-IFN-α, or anti-IFN-β (10 μg/ml each). Transcript levels for Oasl2, Gbp2, Cxcl10, Tyki, Iigp, and Tnfa were normalized to β-actin. Data are pooled from two experiments conducted on separate days (n = 4 per group). Significance determined by 1-way ANOVA followed by Dunnett’s multiple comparison test versus media. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. (B) Flow cytometric analysis of Stat-1 phosphorylation after 15 h stimulation with B. burgdorferi and treatment with blocking antibodies. Data are representative of three independent experiments.