Fig. 3.

IL-2 and IL-7 contribute to Th17 engraftment but IL-15 does not. a Host lymphodepletion improves Th17 engraftment and persistence. Donor Th17 cell (CD4⁺Vb14⁺CD45.2⁺) persistence in peripheral blood (left panel) and spleen at 1 month (right panel) after adoptive transfer into CD45.1⁺ mice with or without 6 Gy total body irradiation 1 day prior to adoptive transfer. Graphs show mean plus/minus standard error of 3–4 mice per group, representative of two independent experiments. ***p < .001 by two-tailed t test assuming equal variance. b IL2Rγ-chain cytokine depletion affects Th17 engraftment. Th17 persistence in the spleen and peripheral blood approximately 1 week (early) or 1 month (late) after adoptive transfer into a lymphodepleted host, with IL2Rγ-chain cytokines being depleted from day 0 to 26 post-adoptive transfer. *p < .05,** p < .01, *** p < .001 for each condition vs vehicle by two-tailed t test. c IL-17 and IFNγ production by Th17 cells before adoptive transfer and after recovery from host spleens (with no cytokine depletion) 1 month post-adoptive transfer. d Graphs show mean plus standard error of cytokine production by donor cells from five mice (Day 27 data), representative of two independent experiments. e Effects of IL2Rγ-chain cytokine depletion on donor Th17 IL-17 and IFNγ production. Mean plus standard error of 4–5 mice per group, representative of two independent experiments