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. Author manuscript; available in PMC: 2018 Oct 1.
Published in final edited form as: Circ Cardiovasc Genet. 2017 Oct;10(5):e001902. doi: 10.1161/CIRCGENETICS.117.001902

Figure 2. Identification of the PRRX1 enhancer underlying the 1q24 AF-association locus.

Figure 2

A. The representative zebrafish for the initial enhancer (E–K) / promoter(Pr) screen displaying the tissue distribution and intensity of the enhancer and promoter activity. Arrows indicate the cardiac region. Scale bar indicates 1mm. B. qRT-PCR results measuring relative eGFP expression levels in zebrafish enhancer/promoter screen (n=3). C. Hi-C data showing organization of topologically associated domains around E and F enhancers in fibroblast cell lines (IMR90) as compared to human embryonic stem cells (hESC). D. Results of chromatin conformation capture (3C) experiment displaying increased frequency of interaction between the E and F enhancers and PRRX1 promoter in human cardiac fibroblasts (HCF)(n=5) as compared to hESC (n=4). *Represents p<0.05.