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. Author manuscript; available in PMC: 2018 Mar 11.
Published in final edited form as: Nat Immunol. 2017 Sep 11;18(11):1238–1248. doi: 10.1038/ni.3835

Fig. 4. Cofactor-binding activity of the FoxP3 mutants.

Fig. 4

After co-transduction of wild-type or mutant FoxP3 (FLAG-tagged) with each co-factor (GFP, HA or 6xHIS-tagged) into HEK293 cells, cell lysates were precipitated with anti-FLAG, and the co-precipitated TFs detected by immunoblot. (a) Representative co-immunoprecipitations. (each row is from a single representative experiment, cut for clarity to remove additional lanes on some gels). (b) Color-coded quantification (average of 2 to 5 such experiments), normalized to co-IP of each TF by wild-type FoxP3. Standard deviation and p-value of co-IP experiments shown in Supplementary Table 6.