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. 2017 Nov 9;6:e22626. doi: 10.7554/eLife.22626

Table 1. Primer and target sequences.

Primers gRNA targets
lefty1 1 CGTGGCTTTCATGTATCACCTTC cxcr4a target 1 G G A CATCGGAGC CAACTTTG
lefty1 1 G CATTAG CCTATATG TTAACTTG CAC. cxcr4a target 2 GTAC CG TCTG CA C CT CTCAG
Iefty2 1 GGGACACAAG CTTTG AAGG G cxcr4a target 3 CG C CTTC ATCA GTTTG G ACC
Iefty2 2 TCCCTGTGTGAGTGAGATCG cxcr4a target 4 CGCCGCGCTCCTCACTGTGC
Iefty2 3 CAGCTGTTCATTTTGACCACTCAC cxcr4a target 5 G GA CTC GTTTG TCACAT G G G
Iefty2 4 AT G GAG CTTC AG C AT G G AC AG sox32 target 1 CGTTCTGATGTTG C AAATAGTGG
aplnrB 1 TGTGTGAATATGATGAGTGGGAAC sox32 target 2 G G CTTAAT G G GCC CGACGCGGGG
aplnrB 2 AGTGGTATCCCAGAGCAGTGTAG sox32 target 3 CC GC G TCG G G CCC ATTAAG CC CG
cxcr4a 1 CGTCTTTGAAGATGATTTATCAGC sox32 target 4 G TT C ATCAT G TG G AC G AAAG AG G
cxcr4a 2 CA C GTAAATG ATG CG G TT G G sox32 target 5 CGAAGTGGTATGATGAAGAGTGG
cxcr4a 3 AG ACT G AA G GAG CTG G AG AAG sox32 target 6 AGTG GAAAC GT GTTTG AT G GT G G
qPCR ta F G AAC CAC AG AG GTG CTC CATATC sox32 target 7 G ACTCT GA GTAAGC AG ACC G TG G
qPCR ta R CTGGTGTTGGAGGTAGTGTTTGTG sox32 target 8 GTAGAGCTCCATGATAGGTGGGG
qPCR leftyl F AAG CT CTAC AAG AAG G CCC C AC ACAAG sox32 target 9 G CGTGTGTG CTGTGTTTG G GTG G
qPCR leftyl R TTCG TG AATG G G AATC AAC CTG G A A
qPCR actin F ATCAGGGTGTCATGGTTGGT
qPCR actin R CACGCAGCTCGTTGTAGAAG
sox32 F AAACTTCTCACG CTT C AC ACC
sox32 R CCATCCAGATTGCTG CTG ATTT