Key resource table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| strain, strain background (Shigella flexneri,serotype 5a strain M90T) |
GFP-tagged shigella | other | Jost Enninga (Paris) | |
| strain, strain background (Shigella flexneri,serotype 5a strain M90T) |
RFP-tagged shigella | other | Jost Enninga (Paris) | |
| strain, strain background (Shigella flexneri,serotype 5a strain M90T) |
mCherry-tagged shigella | PMID:24039575 | strain only used in S. Mostowy lab (London) |
|
| cell line (Homo sapiens) | U2OS | other | Matthias Peter (Zurich) | |
| cell line (H. sapiens) | U2OS-KERMIT | PMID:26259702 | ||
| cell line (Cercopithecus
aethiops) |
COS7 | UCSF cell culture facility | ||
| cell line (H. sapiens) | KERMIT-DRP1CRISPR | this paper | CRISPR-mediated DRP1 knockout cell line, generated using pX330-DRP1e × 2 and pX330- DRP1e × 6 (see entry for these plasmids) |
|
| antibody | Anti-DRP1 (mouse monoclonal) | Abcam | Abcam:ab56788 | (1:2000) |
| antibody | Anti-MFF (rabbit polyclonal) | SIGMA-ALDRICH | SIGMA:HPA010968 | (1:50) |
| antibody | Anti-MFF (rabbit polyclonal) | Protein Tech Group, Inc. | Proteintech: 17090–1-AP |
(1:2000) |
| antibody | Anti-α-Tubulin | SIGMA-ALDRICH | SIGMA:T5168 | (1:2000) |
| recombinant DNA reagent | GFP-DRP1 (plasmid) | PMID:26101352 | ||
| recombinant DNA reagent | Mid49-Cherry (plasmid) | PMID:26101352 | ||
| recombinant DNA reagent | mCherry-DRP1 (plasmid) | PMID:21885730 | ||
| recombinant DNA reagent | mCherry-Lifeact (plasmid) | PMID:22980331 | ||
| recombinant DNA reagent | ATL1-K80A; CLIMP-63 (plasmid) | other | Robin Klemm (Zurich) | |
| recombinant DNA reagent | Cyto-ATL2 (plasmid) | PMID:28826471 | ||
| recombinant DNA reagent | pLVX-puro-GFP-Lifeact; pLVX-puro-RFP-Lifeact; pPax2; pMD2.G (plasmid) |
other | Michael Way (London) | |
| recombinant DNA reagent | pLVX-GFP-Lifeact; pLVX-RFP-Lifeact (plasmid) |
this paper | puromycin-resistant cassette deleted. | |
| recombinant DNA reagent | pLVX-mtBFP (plasmid) | this paper | Progentiors: PCR, mtBFP from pcDNA3.1-mtBFP (PMID:26259702); Vector pLVX |
|
| recombinant DNA reagent | pLVX-Puro-MFF (plasmid) | this paper | Progenitors: PCR, GFP-MFF (Addgene:49153); Vector pLVX-puro |
|
| recombinant DNA reagent | pLVX-mCherry-Fis1TM (plasmid) | this paper | Progenitors: PCR, Fis1 transmembrane domain from pBK416 (PMID:28864540); Vector pLVX |
|
| recombinant DNA reagent | shCtrl (plasmid) | SIGMA-ALDRICH | SIGMA:SHC201 | |
| recombinant DNA reagent | shDRP1 (plasmid) | SIGMA-ALDRICH | SIGMA: TRCN0000318425 |
|
| recombinant DNA reagent | pX330-DRP1e × 2 (plasmid) | this paper | pX330-DRP1e × 2 was generated by cloning the following annealed oligodeoxynucleotides into the pX330 vector (5’-caccGTGACAATTCCAGTACC TCT-3’, 5’-aaacAGAGGTACTGGAATT GTCAC-3’;) |
|
| recombinant DNA reagent | pX330-DRP1e × 6 (plasmid) | this paper | pX330-DRP1e × 6 was generated by cloning the following annealed oligodeoxynucleotides into the pX330 vector (5’- caccGAGACCTCTCATTC TGCAAC-3’, 5’- aaacGTTGCAGAATGAG AGGTCTC-3’) |
|
| sequence-based reagent | siDRP1 #1 | PMID:15286177 | siRNA 5'-UCCGUGAUGAGUAUG CUUUdTdT-3' |
|
| sequence-based reagent | siDRP1 #2 | PMID:21186368 | siRNA 5′-CTGGAGAGGAATGCTGAAA-3′ | |
| sequence-based reagent | siMFF | this paper | siRNA 5′-CUGAGCAGUUCUGCA GUAACAdTdT-3′ |
|
| sequence-based reagent | siINF2-CAAX | PMID:23349293 | siRNA 5′-ACAAAGAAACTGTGTGTGA-3′ | |
| software, algorithm | ndsafir | PMID:19900849 |