Table 2. RNA quality assessment for donors with and without AD in multiple studies.
| Data set | Brain region | Count (Control|AD) |
RIN/pH in control | RIN/pH in AD | P-value |
|---|---|---|---|---|---|
| ACT cohort (current data set) | TCx | 50|29 | 6.87 ± 0.92 | 6.18 ± 1.27 | p=1.48×10−2 |
| ROS (Bennett et al., 2012a) | TCx | 107|136 | 7.21 ± 1.01 | 7.06 ± 0.95 | p=0.23 (ns) |
| MAP (Bennett et al., 2012b) | TCx | 94|120 | 7.25 ± 1.07 | 6.79 ± 0.96 | p=1.18×10−3 |
| MSBB * | BA36 | 98|169 | 6.51 ± 1.30 | 5.86 ± 1.59 | p=1.10×10−3 |
| Mayo Study (Allen et al., 2016) † | TCx | 31|82 | 7.64 ± 1.21 | 8.59 ± 0.55 | p=1.98×10−4 |
| (Colangelo et al., 2002) | CA1 | 6|6 | 6.75 ± 0.1 | 6.76 ± 0.1 | ns |
| (Preece and Cairns, 2003) $ | Cortex | 81|90 | ~6.5 | ~6.4 | p<10−2 |
| (Durrenberger et al., 2010) ‡,$ | Brain | 72|12 | ~7.1 | ~5.9 | p<10−4 |
| (Zhang et al., 2014) | PFc | 101|129 | 7.31 ± 0.47 6.55 ± 0.34 |
7.12 ± 0.56 6.37 ± 0.32 |
p=6.78×10−3
p=1.22×10−4 |
*=we are defining AD as CDR >1 in this data set, but note that the result holds for other cutoffs, †=only control donors from the Mayo Brain Bank Dickson were considered, ‡=Tissue collected from multiple brain regions and multiple brain banks. RNA quality is assessed with either RIN (black text) or pH (blue text) in each study. $=RIN/pH values are estimated from plots. Calculated p-values in this table are two tailed student t-tests uncorrected for multiple comparisons, and p-values from previous studies are as reported.